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Molecular Formula: C9H6O4
Molecular Weight: 178.14
EINECS: 207-618-1
Purity: 97% Min.
Package: 5 g / 25 g / 1 kg / 25 kg
CAS: 485-47-2 Ninhydrin hydrate
Ninhydrin is historically the most common reagent for processing fingerprints on porous substrates. It has been used as a fingerprint reagent since the 1950s but has been used as a cell stain since the early 1900s. Ninhydrin molecules form a complex with amino acids resulting in a purple product known as Ruhmann's purple. Purple fingerprints are easily visible on light-colored porous substrates but may be difficult to see on patterned or dark-colored substrates.
DFO reacts with amino acids to produce a fluorescent product that is not readily seen with the naked eye. This makes it a useful reagent on dark or patterned porous items. DFO prints are viewed with an alternate light source or laser set to 530¨C570 nm (green light) through a red barrier filter. 1,2-indanedione is an emerging fingerprint reagent that results in visible pink fingerprints that are also fluorescent. The fluorescence is viewed under the same conditions as DFO.
SPECIFICATION OF CAS: 485-47-2 Ninhydrin hydrate
ITEMS | SPECIFICATION |
solubility | ethanol |
color | white to brownish-white, crystals |
Boiling point | 230.35¡ãC (rough estimate) |
Specific Gravity | 0.788 |
pka | 8.47¡À0.20(±Ê°ù±ð»å¾±³¦³Ù±ð»å) |
BRN | 1910963 |
storage temp. | Keep in dark place,Inert atmosphere,Room temperature |
refractive index | 1.4209 (estimate) |
form | Powder/Solid |
PH Range | 4.6 - 5.0 |
PH | 4.6-5.0 (10g/l, H2O, 20¡æ) |
InChIKey | FEMOMIGRRWSMCU-UHFFFAOYSA-N |
Fp | 13&²Ô²ú²õ±è;¡ã°ä |
EPA Substance Registry System | Ninhydrin (485-47-2) |
density | 0.862 |
Water Solubility | 0.1-0.5 g/100 mL at 20 ?C |
vapor density | 6.16 (vs air) |
Melting point | 250 ¡ãC (dec.) (lit.) |
Merck | 14,6554 |
Stability | Stable. Incompatible with amines, alkalies, strong bases. Light-sensitive. |
Sensitive | Light Sensitive |
NIST Chemistry Reference | Ninhydrin(485-47-2) |
CAS DataBase Reference | 485-47-2(CAS DataBase Reference) |
Odor | Odorless |
Application:
Ninhydrin is an amino acid-sensitive reagent for the development of latent fingermarks on porous surfaces. More sensitive alternatives, such as DFO and IND-Zn, are now available and these have advantages on porous substrates that are not highly luminescent. However, ninhydrin can still be applied after these treatments and may reveal additional ridge detail, particularly on highly luminescent substrates where luminescence visualization may be problematic.
Reactions:
Ninhydrin reacts with primary and secondary amines (including amino acids, proteins, and peptides) to give a dark purple product known as Ruhemann's purple (RP) . As the eccrine component of a latent mark deposit contains amino acids, this reaction can be exploited as a means of developing fingermarks on porous surfaces such as paper and cardboard. The use of ninhydrin as a fingermark detection reagent was first proposed in 1954 by Od¨¦n and von Hofsten (1954). Since then, ninhydrin has become the most popular technique for fingermark detection on porous substrates. Eccrine glands secrete a range of different amino acids that may ultimately be present in a latent fingermark deposit (Hamilton 1965; Ramotowski 2001). Ninhydrin is a nonspecific amino acid reagent in that it reacts in the same manner with different amino acids. In this way, each amino acid present in the latent fingermark deposit will contribute to the developed fingermark image. Amino acids are stable compounds that, due to an affinity for cellulose, do not migrate to any significant extent through dry paper substrates. As a result, very old latent marks can be developed with ninhydrin (the development of 40-year-old marks has been recorded), and the revealed marks are normally of good quality. In addition, the amino acid composition of the eccrine secretion appears to remain relatively constant. Due to these qualities, the use of amino acid reagents (ninhydrin and ninhydrin analogs, including 1,8-diazafluoren-9-one [DFO]) constitutes an effective chemical technique for the development of latent fingermarks on paper surfaces.
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